home-cage activity observation system phenomaster Search Results


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TSE systems phenomaster home cage system
A mRNA expression in wild‐type mice without treatment, as determined by qRT–PCR ( n = 6). scWAT: subcutaneous white adipose tissue; gWAT: gonadal WAT; isBAT: interscapular brown adipose tissue; SKM: gastrocnemius skeletal muscle. 2 × 2 ANOVA with Holm–Sidak posttests, *** P = 0.0004 (female vs. male), ### P = 0.0004, ### P = 7 × 10 −6 , ### P = 6 × 10 −9 , ### P = 3 × 10 −10 , (isBAT, SKM, heart, liver, respectively, vs. scWAT within indicated sex), # P = 0.032 (females: gWAT vs. scWAT), ## P = 0.006 (females: Heart vs. scWAT), ## P = 0.006 (males: Liver vs. scWAT). B Ucp1 expression in scWAT of mice fed a diet with 0.0075% Rosi or control diet for 2.5 weeks, determined by Western blot with VCP as loading control ( n = 3, same samples as in Fig C and D). C mRNA expression in BAT of mice treated as in (A), determined by qRT–PCR. t ‐test Cited4 −/− vs. Cited4 +/+ (Rosi), females: n = 5/5/6/6, males: n = 5/4/5/5, *** P = 0.0004. D mRNA expression in gWAT of mice treated as in (A), determined by qRT–PCR. t ‐test Cited4 −/− vs. Cited4 +/+ (Rosi), females: n = 5/5/6/6, males: n = 5/4/5/5. E Three‐day averages of total activity counts per day and mouse of female mice fed a diet with 0.0075% Rosi for 2.5 weeks, determined by <t>Phenomaster</t> ( n = 9/10, t ‐test). F Three‐day averages of food intake per day and mouse of female mice shown in (E) ( n = 9/10, t ‐test). G Oxygen consumption rate of female mice fed control diet, determined by indirect calorimetry and adjusted for body weight by ANCOVA. Three‐day averages of VO 2 were calculated for each mouse ( n = 10). ANCOVA with Bonferroni's posttest ( Cited4 −/− vs. Cited4 +/+ ). Data information: Data are presented as mean ± SEM. Source data are available online for this figure.
Phenomaster Home Cage System, supplied by TSE systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A mRNA expression in wild‐type mice without treatment, as determined by qRT–PCR ( n = 6). scWAT: subcutaneous white adipose tissue; gWAT: gonadal WAT; isBAT: interscapular brown adipose tissue; SKM: gastrocnemius skeletal muscle. 2 × 2 ANOVA with Holm–Sidak posttests, *** P = 0.0004 (female vs. male), ### P = 0.0004, ### P = 7 × 10 −6 , ### P = 6 × 10 −9 , ### P = 3 × 10 −10 , (isBAT, SKM, heart, liver, respectively, vs. scWAT within indicated sex), # P = 0.032 (females: gWAT vs. scWAT), ## P = 0.006 (females: Heart vs. scWAT), ## P = 0.006 (males: Liver vs. scWAT). B Ucp1 expression in scWAT of mice fed a diet with 0.0075% Rosi or control diet for 2.5 weeks, determined by Western blot with VCP as loading control ( n = 3, same samples as in Fig C and D). C mRNA expression in BAT of mice treated as in (A), determined by qRT–PCR. t ‐test Cited4 −/− vs. Cited4 +/+ (Rosi), females: n = 5/5/6/6, males: n = 5/4/5/5, *** P = 0.0004. D mRNA expression in gWAT of mice treated as in (A), determined by qRT–PCR. t ‐test Cited4 −/− vs. Cited4 +/+ (Rosi), females: n = 5/5/6/6, males: n = 5/4/5/5. E Three‐day averages of total activity counts per day and mouse of female mice fed a diet with 0.0075% Rosi for 2.5 weeks, determined by Phenomaster ( n = 9/10, t ‐test). F Three‐day averages of food intake per day and mouse of female mice shown in (E) ( n = 9/10, t ‐test). G Oxygen consumption rate of female mice fed control diet, determined by indirect calorimetry and adjusted for body weight by ANCOVA. Three‐day averages of VO 2 were calculated for each mouse ( n = 10). ANCOVA with Bonferroni's posttest ( Cited4 −/− vs. Cited4 +/+ ). Data information: Data are presented as mean ± SEM. Source data are available online for this figure.

Journal: EMBO Molecular Medicine

Article Title: Cited4 is a sex‐biased mediator of the antidiabetic glitazone response in adipocyte progenitors

doi: 10.15252/emmm.201708613

Figure Lengend Snippet: A mRNA expression in wild‐type mice without treatment, as determined by qRT–PCR ( n = 6). scWAT: subcutaneous white adipose tissue; gWAT: gonadal WAT; isBAT: interscapular brown adipose tissue; SKM: gastrocnemius skeletal muscle. 2 × 2 ANOVA with Holm–Sidak posttests, *** P = 0.0004 (female vs. male), ### P = 0.0004, ### P = 7 × 10 −6 , ### P = 6 × 10 −9 , ### P = 3 × 10 −10 , (isBAT, SKM, heart, liver, respectively, vs. scWAT within indicated sex), # P = 0.032 (females: gWAT vs. scWAT), ## P = 0.006 (females: Heart vs. scWAT), ## P = 0.006 (males: Liver vs. scWAT). B Ucp1 expression in scWAT of mice fed a diet with 0.0075% Rosi or control diet for 2.5 weeks, determined by Western blot with VCP as loading control ( n = 3, same samples as in Fig C and D). C mRNA expression in BAT of mice treated as in (A), determined by qRT–PCR. t ‐test Cited4 −/− vs. Cited4 +/+ (Rosi), females: n = 5/5/6/6, males: n = 5/4/5/5, *** P = 0.0004. D mRNA expression in gWAT of mice treated as in (A), determined by qRT–PCR. t ‐test Cited4 −/− vs. Cited4 +/+ (Rosi), females: n = 5/5/6/6, males: n = 5/4/5/5. E Three‐day averages of total activity counts per day and mouse of female mice fed a diet with 0.0075% Rosi for 2.5 weeks, determined by Phenomaster ( n = 9/10, t ‐test). F Three‐day averages of food intake per day and mouse of female mice shown in (E) ( n = 9/10, t ‐test). G Oxygen consumption rate of female mice fed control diet, determined by indirect calorimetry and adjusted for body weight by ANCOVA. Three‐day averages of VO 2 were calculated for each mouse ( n = 10). ANCOVA with Bonferroni's posttest ( Cited4 −/− vs. Cited4 +/+ ). Data information: Data are presented as mean ± SEM. Source data are available online for this figure.

Article Snippet: Oxygen consumption (indirect calorimetry), food intake, and locomotor activity measurements were performed with individually housed mice at 22°C in the PhenoMaster Home Cage System (TSE system, Bad Homburg, Germany).

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot, Activity Assay